Human TGF alpha ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH188
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
Transforming Growth Factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF alpha (TGFA) shows about 40% sequence homology with Epidermal Growth Factor (EGF) and competes with EGF for binding to the EGF Receptor (EGFR), stimulating its phosphorylation and producing a mitogenic response. The Human TGF alpha ELISA is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF alpha in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human TGF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human TGF alpha antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF alpha bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
Sensitivity
The minimum detectable dose of TGF alpha is typically less than 3 pg/mL.

Arbeitsanleitung

CS-CKH188.pdf

Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €
lieferbar

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