Human TGF-beta 2 ELISA Kit

Hersteller Cell Sciences
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH190
eClass 6.1 32160605
eClass 9.0 32160605
Transforming Growth Factor beta exists in at least five isoforms, known as TGF beta 1, TGF beta 2, TGF beta 3, TGF beta 4, TGF beta 5. Their amino acid sequences display homologies on the order of 70-80%. The various TGF beta isotypes share many biological activities and their actions on cells are qualitatively similar in most cases although there are a few examples of distinct activities. TGF beta 2 is the only variant that does not inhibit the growth of endothelial cells. TGF beta 2 and TGF beta 3 inhibit the survival of cultured embryonic chick ciliary ganglionic neurons. The Human TGF beta 2 ELISA is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 2 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human TGF beta 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human TGF beta 2 antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 2 bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
The minimum detectable dose of TGF beta 2 is typically less than 15 pg/mL.
Menge: 1 Plate
Lieferbar: Out of stock
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