Human Endostatin ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH334
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Description
Endostatin is found in the conditioned medium of the hemangioendothelioma cell line EOMA. It has been identified as a fragment of the nontriplehelical C-terminal NC1 domain of collagen type 18 and has been referred to as endostatin 18. Endostatin specifically inhibits proliferation of endothelial cells although it does not affect the proliferationof EOMA cells. It also potently inhibits angiogenesis and tumor growth.The Cell Sciences Human Endostatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Endostatin in serum, plasma, cell culture supernants and urine. This assay employs an antibody specific for human Endostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Endostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Endostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endostatin bound. The Stop Solution changes the color from blue to yellow, and theintensity of the color is measured at 450 nm
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 786,58 €
Preis: 786,58 €
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