Mouse G-CSF/CSF3 ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Mouse
Menge 1 Plate
ArtNr CS-CK423
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
G-CSF is an O-glycosylated glycoprotein, which is secreted by monocytes, macrophages and neutrophils. G-CSF is also produced by stromal cells, fibroblasts, and endothelial cells. Epithelial carcinomas, acute myeloid leukemia cells and various tumor cell lines, also express this factor. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage. It is a mitogen for some mouse myeloid leukemia cells and also for some carcinoma cell lines. In vitro G-CSF enhances the antibody-dependent cellular cytotoxicity of granulocytes against tumor cells.The Cell Sciences Mouse G-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse G-CSF in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for mouse G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Arbeitsanleitung

CS-CK423.pdf

Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €
lieferbar

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