Mouse IL-4 ELISA Kit

Hersteller Cell Sciences
Kategorie Produkte / ELISA
Typ Elisa-Kit
Specific against Mouse
Menge 1 Plate
ArtNr CS-CK429
Eclass 6.1 32160605
Eclass 9.0 32160605
Mouse Interleukin-4 (IL4) is a pleiotropic cytokine produced by activated T-cells and is a ligand for Interleukin-4 Receptor (IL4R). IL4R also binds to IL13, which may contribute to many overlapping functions of IL4 and IL13. Signal Transducer and Activator of Transcription 6 (STAT6) has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL4 gene particularly close to IL13. It is a 20 kDa single chain glycoprotein synthesized from 140 amino acids. IL4 enhances expression of class II MHC antigens on B-cells. It can promote their capacity to respond to other B-cell stimuli and to present antigens for T-cells. IL4 inhibits cell activation of NK-cells induced by IL2. It stimulates the proliferation of thymocytes with the marker spectrum CD4(-)CD8(-), CD4(+)CD8(-), and CD4(-)CD8(+). The Mouse IL4 ELISA is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL4 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for IL4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Mouse IL4 antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL4 bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
The minimum detectable dose of IL4 is typically less than 0.6 pg/mL.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 693,01 €
Preis: 693,01 €

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Salesmanager: Arne Pelz