Mouse MCP1 ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Mouse
Menge 1 Plate
ArtNr CS-CK433
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
Monocyte chemoattractant protein-1 (MCP-1), also called monocytes chemotacticprotein-1, belongs to the family of Chemokines. MCP-1 is expressed by monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cell, andosteoblastic cells. It has been shown to exhibit biological activities other than Chemotaxis. The activity of mouse MCP-1 has been shown to be mediated by the mouse CC chemokine receptor CCR2.The Cell Sciences Mouse MCP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP-1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse MCP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Arbeitsanleitung

CS-CK433.pdf

Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €
lieferbar

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