Mouse Leptin ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Mouse
Menge 1 Plate
ArtNr CS-CKM056
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
Leptin (LEP) is a 16 kDa protein secreted by white adipocytes that plays a major role in the regulation of body weight. LEP acts through the Leptin Receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. LEP also has thermogenic actions and regulates enzymes of fatty acid oxidation. LEP has several endocrine functions as well, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in the LEP gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. LEP has also been linked to Type 2 Diabetes Mellitus development. Human and mouse LEP show approximately 84% identity at the protein level. Studies indicate that Human obesity may be associated with Leptin Receptor deficiencies rather than constituting a problem with LEP itself. The Human LEP ELISA is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human LEP in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for LEP coated on a 96-well plate. Standards and samples are pipetted into the wells and LEP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human LEP antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LEP bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zip seal.
Sensitivity
The minimum detectable dose of LEP is typically less than 1.5 pg/ml.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 693,01 €
Preis: 693,01 €
lieferbar

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Arbeitsanleitung

CS-CKM056.pdf