Mouse Thrombopoietin ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Mouse
Menge 1 Plate
ArtNr CS-CKM065
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
Megakaryocytopoiesis is the cellular development process that leads to platelet production. Thrombopoietin (THPO) is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. THPO is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. The Mouse THPO ELISA is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Mouse THPO in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for THPO coated on a 96-well plate. Standards and samples are pipetted into the wells and THPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Mouse THPO antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of THPO bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
Sensitivity
The minimum detectable dose of THPO is typically less than 30 pg/mL.

Arbeitsanleitung

CS-CKM065.pdf

Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €
lieferbar

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