Rat IL-6 ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Rat
Menge 1 x 96 tests
ArtNr CS-CKR056
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Description
The Rat IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-6 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for rat IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage and Stability
May be stored for up to 6 months at 2-4C from the date of shipment. Standard should be stored at -80C after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. The kit can be used within 1 year if it is stored at -20C. Avoid repeated freeze-thaw cycles.
Range
246 - 60, 000 pg/ml
Sensitivity
The minimum detectable dose of IL-6 is typically less than 200 pg/ml.
Cross-Reactivity
This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1a, IL-1a, IL-4, IL-10, GM-CSF, IFN-a, Leptin, Lix, MCP-1, MIP-3a, a-NGF, TIMP-1, TNF-a.
Menge: 1 x 96 tests
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €
lieferbar

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