Recombinant Bovine FGF basic

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Bovine
Menge 1.0 mg
Host E.coli
ArtNr CS-CRF023C
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Fibroblast Growth Factor-basic (bFGF) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor.Other homologous FGF belonging to the same family are int-2 ( FGF-3 ), FGF-5, FGF-6, K-FGF and KGF ( keratinocyte growth factor, FGF-7 ). All factors are products of different genes, some of which are Oncogene products (FGF-3, FGF-4, FGF-5).
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
Stable at 2-8C, but best kept desiccated -20C. Upon reconstitution, stable for up to 1 week at 2-8C. For longer term, store in working aliquots below -20C. Avoid repeated freeze/thaw cycles.
GeneID
281161
Source
E. coli
Molecular Weight
Approximately 16.5 kDa
Purity
>97% by SDS-PAGE and HPLC analyses.
Specific Activity
1.0 x 107 Units/mg
Endotoxin Level
Less than 1EU/ug as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using mouse balb/c 3T3 cells is less than 0.1 ng/ml.
AA Sequence
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS
Menge: 1.0 mg
Lieferbar: In stock
Listenpreis: 1.448,53 €
Rabatt: -10,0%
Preis: 1.303,68 €
Sie sparen: 144,85 €
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Arbeitsanleitung

CS-CRF023C.pdf