Native Chicken Filamin

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Gallus
Menge 10 ug
Host Chicken
ArtNr CS-CRF123A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Filamin is a large (270 kd) dimeric actin cross linking protein from a variety of sources, which helps to stabilize the 3D cortical actin network. The fundamental structure of filamin is well conserved and consists of an actin binding domain at the N-terminus followed by a C-terminal rod domain consisting of numerous repeat segments ranging from 4 in C. elegans to 24 in mammalian cells. Each repeat in the rod domain consists of roughly 100 residues and forms an immunoglobulin like fold. Such immunoglobulin folds have been found in a variety of proteins and are responsible for protein-protein interactions. Filamin Human actin-binding protein (ABP), aka filamin, crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Mammalian filamin interacts directly with at least 30 proteins such as transmembrane receptors, second messenger-associated proteins, protein kinases, phosphatases and cytoskeletal proteins and these interactions have been shown to require one or more of the repeat elements in the rod domain.
Formulation
The protein was lyophilized from a 1mg/ml solution containing 20 mM Tris / acetate buffer pH 7.6 + 0.1 mM EDTA, +2 mM DTT and 20 mM NaCl. Each mg Filamin contains 550 mg urea.
Storage and Stability
Lyophilized Filamin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Filamin should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Source
Chicken Gizzard.
Molecular Weight
Ultra Pure Filamin having a MW = 250 kDa.
Purity
Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Menge: 10 ug
Lieferbar: In stock
Listenpreis: 226,60 €
Preis: 226,60 €
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