Recombinant Human Artemin

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 1.0 mg
Host E.coli
ArtNr CS-CRA001C
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Artemin is a novel member of the glial cell line-derived neurotrophic factor (GNDF) ligand family. Current evidence suggests that Artemin signals through the receptor complex, GFRalpha3-RET, to influence neuron survival, in vitro, and, in vivo . Recombinant human Artemin is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains.
Formulation
Recombinant Artemin is lyophilized with 10 mM sodium citrate pH 4.5 and 25 mM NaCl.
Reconstitution
Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Storage and Stability
Lyophilized product is very stable at -20C. Reconstituted material should be aliquoted and frozen at -20C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
GeneID
9048
Source
E. coli
Molecular Weight
24.2 kDa
Purity
98.0% as determined by Reducing and Non-reducing SDS-PAGE, HPLC
Protein Accession No
Q5T4W7
Endotoxin Level
Measured by kinetic LAL analysis and is typically <= 1 EU/ug protein.
Biological Activity
The activity is determined by the dose-dependant proliferation of the SH-SY5Y cell line and is typically 4-8 ng/mL. The activity can also be determined by its ability to promote survival and neurite outgrowth.
AA Sequence
AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG
Menge: 1.0 mg
Lieferbar: In stock
Listenpreis: 3.036,76 €
Rabatt: -10,0%
Preis: 2.733,08 €
Sie sparen: 303,68 €
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Arbeitsanleitung

CS-CRA001C.pdf