Recombinant Human *diponectin/Acrp30 Flag

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 1.0 mg
Host HEK293 cells
ArtNr CS-CRA026C
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Recombinant human Adiponectin fused with FLAG tag.
Formulation
Lyophilized from a 0.4 uM filtered solution in 20 mM Tris Buffer + 20 mM NaCl, pH 7.5.
Reconstitution
Centrifuge vial prior to opening. Add 0.2 ml sterile distilled water to the vial to fully solubilize the protein to a concentration of 0.5 mg/ml. Note: Product not sterile. Please filter product by an appropriate sterile filter before using it in cell culture.
Storage and Stability
Lyophilized protein is stable for 2 years at -20C and reconstituted protein is stable for 2 weeks at 2-4C. Avoid repeated freeze-thaw cycles.
GeneID
9370
Source
HEK 293 (Human embryonic kidney cell line)
Purity
> 98% as determined by HPLC and SDS-PAGE analyses
Endotoxin Level
< 0.1 ng/ug of ACRP30
Biological Activity
in vitro, gluconeogenesis assay in primary hepatocytes was performed, showing the Adiponectin human derived from mammalian cells can inhibit glucose production. The ED50 was ca.6 ug/ml.
AA Sequence
ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDD DDK
Menge: 1.0 mg
Lieferbar: Out of stock
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