Recombinant Human AKT1/PKB (aa 1-480) His Active *di

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 10 ug
ArtNr CS-CRA153A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description
Recombinant Human Active AKT1, full length (aa 1-480) with N-terminal HIS tag, co-expressed with PDK1.
Formulation
Sterile filtered liquid in a buffer of 25 mM Tris-HCl, pH 8.0 + 100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 3 mM DTT.
Storage and Stability
Centrifuge vial prior to opening . Stable for >6 months at -80C.
Source
Sf9 cells
Molecular Weight
60.2 kDa
Purity
>70% as determined by SDS-PAGE analysis.
Biological Activity
12.32 pmol/min/ug enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.5 + 10 mM MgCl2 + 1 mM EGTA + 200 uM ATP + 0.01% Brij-35 + 2 uM substrate at room temperature for 1 hour.
Menge: 10 ug
Lieferbar: In stock
Listenpreis: 543,94 €
Preis: 543,94 €
lieferbar

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