Recombinant Human GITRL His

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 5 ug
Host E.coli
ArtNr CS-CRA300A
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Osteostat is the cytokine that binds to TNFRSF18/AITR/GITR, is important for interactions between activated T-lymphocytes and endothelial cells, and may modulate T-lymphocyte survival in peripheral tissues. Osteostat is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells after stimulation by lipopolysaccharides. Osteostat protein is detectable in human microvascular EC and is highly up-regulated by IFN-alpha and IFN-beta. Osteostat inhibits differentiation of osteoclasts from monocytic precursor cells. Osteostat suppresses the early stage of osteoclastogenesis via inhibition of macrophage colony-stimulating factorinduced receptor activator of NF-kappaB (RANK) expression in the osteoclast precursor cells. Osteostat does not inhibit lipopolysaccharide-induced RANK expression in monocytes and dendritic cells, or activation-induced RANK expression in T cells. Osteostat is a novel regulator of osteoclast generation and substantiate the major role played by the endothelium in bone physiology. Recombinant human TNFSF18 with His Tag contains 125 amino acid residues of human TNFSF18 and 14 additional amino acid residue-His Tag. The amino acid sequence of recombinant human TNFSF18 is homologous to the extracellular domain of human TNF18, Thr53-Ser177.
Formulation
Lyophilized from a sterile filtered solution containing 0.03 M acetate buffer, pH 4.0
Reconstitution
Centrifuge vial prior to opening. Add 0.2 ml of 0.1 M acetate buffer, pH 4.0 to the vial to fully solubilize the protein. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ug/ml. In higher concentrations the solubility of this antigen is limited.
Storage and Stability
Store lyophilized protein at -20C to -80C. Reconstituted protein is stable for 1 week at 2-4C. For long term storage, aliquot and store at -20C to -80C with a carrier protein (0.1% HSA or BSA) as a stabilizer. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
GeneID
8995
Source
E. coli
Molecular Weight
15.7 kDa
Purity
> 95% as determined by RP-HPLC and SDS-PAGE analyses
Endotoxin Level
< 0.1 ng/ug of TNFSF18
AA Sequence
MRGSHHHHHH GMASTAKEPC MAKFGPLPSK WQMASSEPPC VNKVSDWKLE ILQNGLYLIY GQVAPNANYNDVAPFEVRLY KNKDMIQTLT NKSKIQNVGG TYELHVGDTI DLIFNSEHQV LKNNTYWGII LLANPQFIS
Menge: 5 ug
Lieferbar: In stock
Listenpreis: 206,00 €
Rabatt: -10,0%
Preis: 185,40 €
Sie sparen: 20,60 €
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Arbeitsanleitung

CS-CRA300A.pdf