Recombinant Human BRAF Active His *di

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 10 ug
ArtNr CS-CRB004
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description
Recombinant Human BRAF (aa 432-725) with N-terminal His tag, coexpressed with p50.
Formulation
Liquid in 25 mM Tris-HCl, pH 8.0 + 150 mM NaCl + 0.05% Tween-20 + 40% glycerol + 3 mM DTT
Storage and Stability
Stable for 6 months at -80C. Avoid repeated freeze-thaw cycles .
Source
Sf9 cells
Molecular Weight
36 kDa
Specific Activity
Activity was tested using 1 uM MEK1 (K97R) as substrate. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 1 uM ATP. The BRAF enzyme at concentration 4 nM phosphorylates about 10% of 1 uM MEK1 at 30C for 120 min.
Protein Accession No
NP_004324
Menge: 10 ug
Lieferbar: In stock
Listenpreis: 538,60 €
Rabatt: -10,0%
Preis: 484,74 €
Sie sparen: 53,86 €
lieferbar

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Arbeitsanleitung

CS-CRB004.pdf