Recombinant Human BCL2 minus BH2 domain His

Hersteller Cell Sciences
Typ Proteins
Specific against Human
Menge 2 ug
Host E.coli
ArtNr CS-CRB130A
eClass 6.1 34160400
eClass 9.0 42020190
BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. Bcl-2 Des BH2 domain (187-202 residues) Human Recombinant produced in, E. coli, is a single, non-glycosylated polypeptide chain containing 202 amino acids 1-186 and 203-218. The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.
Lyophilized from a sterile filtered solution containing 10 mM Tris-HCl, pH 8.0 + 1 mM EDTA + 250 mM NaCl
Centrifuge vial prior to opening. Suspend BCL2 in 100 ul of 0.5 M Acetic acid, over night at 2-4C. Dilute 10 fold into selected buffer system. Because BCL2 has a tendency to form intramolecular disulfide bond, 5 mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10 mM DTT is recommended.
Storage and Stability
Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -20C. Upon reconstitution Bcl-2 should be stored at 2-4C between 2-7 days and for future use below -20C. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
E. coli
> 95% as determined by RP-HPLC and SDS-PAGE analyses
Endotoxin Level
< 0.1 ng/ug of BCL2
Menge: 2 ug
Lieferbar: Out of stock
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