Recombinant Human BAFF

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 5 ug
Host E.coli
ArtNr CS-CRB300A
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Recombinant Human BAFF is a single, non-glycosylated polypeptide chain containing 153 amino acids. B-cell activating factor (BlyS), also known as BAFF, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
GeneID
10673
Source
E. coli
Molecular Weight
17.0 kDa
Purity
>95% as determined by RP-HPLC and SDS-PAGE
Specific Activity
> 5.0 x 10E5 IU/mg in the presence of goat anti-mouse IgM u chain.
Endotoxin Level
Less than 1EU/ug as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using anti-IgM stimulated mouse B cells is less than 2 ng/ml.
AA Sequence
MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL

Arbeitsanleitung

CS-CRB300A.pdf

Menge: 5 ug
Lieferbar: In stock
Listenpreis: 226,60 €
Preis: 226,60 €
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Ihr Ansprechpartner:

Arne Pelz

sales@hoelzel.de