Recombinant Human beta-Defensin 2

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 5 ug
Host E.coli
ArtNr CS-CRB500A
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. beta-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human beta-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because beta-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Recombinant Human Defensin beta 2 is a single non-glycosylated polypeptide chain containing 41 amino acids.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, + 130 mM NaCl.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
The lyophilized protein is stable at 2-8C. Upon receipt, store desiccated at -20C. After reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.
GeneID
1673
Source
E. coli
Molecular Weight
ca.4.3 kDa
Purity
>98% as determined by SDS-PAGE and HPLC analyses.
Endotoxin Level
<1 EU/ug as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration of 10-100 ng/ml.
AA Sequence
GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P
Menge: 5 ug
Lieferbar: In stock
Listenpreis: 206,00 €
Rabatt: -10,0%
Preis: 185,40 €
Sie sparen: 20,60 €
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Arbeitsanleitung

CS-CRB500A.pdf