Recombinant Human Collagen

Hersteller Cell Sciences
Kategorie Produkte / Proteins
Typ Proteins
Specific against Human
Menge 500 mg
Host E.coli
ArtNr CS-CRC000B
Eclass 6.1 34160400
Eclass 9.0 42020190
Recombinant Human Collagen is a non-glycosylated polypeptide chain containing human collagen’s cDNA transcribed reversely from mRNA.
Lyophilized from a sterile solution containing no additives
Centrifuge vial prior to opening. First add sterile water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.
Storage and Stability
Stable at room temperature for 3 weeks, but best kept desiccated below -20C. Upon reconstitution, store at 2-8C for one week and for future use below -20C. For long term storage it is recommended to add a carrier protein. Avoid repeated freeze-thaw cycles.
E. coli
Molecular Weight
96 kDa
>95.0% by RP-HPLC and SDS-PAGE
Biological Activity
Cultivation of BHK-21 and 2BS cell line: BHK-21 or 2BS cells were plated onto the bottom of the 96-well cell culture plates at the density of 1 x 103cells/well, respectively, with 0.1ml RPMI1640 contained 10% fetal bovine serum in the well. BHK-21 or 2BS cells were incubated in standard conditions (at 37C with 5% CO2 in a humidified incubator) for 24 hours.
AA Sequence
The sequence of the first fifteen N-terminal amino acids is MDPVVLQRRDWENPG. The amino acid percentage is as follows: Asp 0.39, Thr 0.16, Ser 5.70, Pro 18.87, Gly 29.17, Ala 13.38, Val.71, Met.18, Ile 0.16, Leu 8.04, Tyr 0.90, Phe 0.15, Lys 0.27, His 0.15, Arg 0.03, Gln 17.40, Hydro Pro 4.34.
Menge: 500 mg
Lieferbar: Out of stock
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