Recombinant Human CDC42BPB/MRCKB GST-His Active

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
ArtNr CS-CRC025
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Recombinant human CDC42BPB containing amino acids M1-H472 (as in GenBank entry NM_006035)*, N-terminally fused to GST-HIS6-Thrombin cleavage site. *Sequence may contain documented polymorphisms. Detailed sequence available upon request.
Formulation
Liquid in 50 mM Tris-HCl, pH 8.0 + 100 mM NaCl + 5 mM DTT + 4 mM reduced glutathione + 20% glycerol
Purification
Affinity purified using GSH-agarose
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Source
Sf9 cells
Molecular Weight
84.4 kDa
Specific Activity
14 pmol/ug x min Method for determination of Km value & Specific activity: Assay conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate, 1.2 mM DTT, 2.5 ug / 50 ul PEG20.000, ATP (variable), Substrate: hum. INSR-rel. receptor (917-923) derived peptide (KKRNRTLTK) 1 ug / 50 ul, Recombinant CDC42BPB: 50 ng / 50 ul Filter binding assay, MSPH membrane (Millipore)
Protein Accession No
NP_006026

Arbeitsanleitung

CS-CRC025.pdf

Menge: 50 ug
Lieferbar: Out of stock
nicht lieferbar

fragen Sie nach einem alternativen Produkt

Fragen zum Produkt?

Ihr Ansprechpartner:

Arne Pelz

sales@hoelzel.de