Recombinant Human Cyclin-Dependent Kinase 1/Cyclin B complex GST-His Active

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
ArtNr CS-CRC026
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Coexpression of human CDC2, amino acids M1 - M297 (as in GenBank entry NM_001786)* and human CCNB1, amino acids M1 - V433 (as in GenBank entry NM_031966)*, both N-terminally fused to GST-HIS6-Thrombin cleavage site. *Sequence may contain documented polymorphisms. Detailed sequence available upon request.
Formulation
Liquid in 50 mM Tris-HCl, pH 8.0 + 100 mM NaCl + 5 mM DTT + 15 mM reduced glutathione + 20% glycerol
Purification
Affinity purified using GSH-agarose
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Source
Sf9 cells
Molecular Weight
GST-CDC2: 63.9 kDa GST-CCNB1: 78.9 kDa
Specific Activity
51 pmol/ug x min Method for determination of Km value & Specific activity: Assay conditions: 60 mM HEPES-NaOH, pH 7.5 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate 1.2 mM DTT 2.5 ug / 50 ul PEG20.000 ATP (variable) Substrate: Rb-CTF, 5 ug / 50 ul Recombinant CDC2/CCNB1: 200 ng / 50 ul Filter binding assay MSFC membrane (Millipore)
Menge: 50 ug
Lieferbar: Out of stock
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Arbeitsanleitung

CS-CRC026.pdf