Recombinant Human Cyclin-Dependent Kinase 1/Cyclin E complex GST-His Active

Hersteller Cell Sciences
Typ Proteins
Specific against Human
Menge 50 ug
ArtNr CS-CRC027
eClass 6.1 34160400
eClass 9.0 42020190
Coexpression of human CDC2, amino acids M1-M297 (as in GenBank entry NM_001786)* and human CCNE1, amino acids M1-A395 (as in GenBank entry M73812)*, N-terminally fused to GST-Thrombin cleavage site. *Sequence may contain documented polymorphisms. Detailed sequence available upon request.
Liquid in 50 mM Tris-HCl, pH 8.0 + 100 mM NaCl + 5 mM DTT + 4 mM reduced glutathione + 20% glycerol
Affinity purified using GSH-agarose
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Sf9 cells
Molecular Weight
GST-CDC2: 63.9 kDa GST-CCNE1: 72.0 kDa
Specific Activity
53 pmol/ug x min Method for determination of Km value & Specific activity: Assay conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate, 1.2 mM DTT, 2.5 ug / 50 ul PEG20.000, ATP (variable), Substrate: Rb-CTF, 5 ug / 50 ul, Recombinant CDC2/CCNE1: 200 ng / 50 ul Filter binding assay, MSFC membrane (Millipore)
Menge: 50 ug
Lieferbar: Out of stock
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