Recombinant Human CDK2/CycA Kinase Complex GST-His Active

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
Host Insect
ArtNr CS-CRC029
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Coexpression of human CDK2, amino acids M1-L298 (as in GenBank entry NM_001798)* and human CCNA2, amino acids M1-L432 (as in GenBank entry NM_001237)*, both N-terminally fused to GST-Thrombin cleavage site. *Sequence may contain documented polymorphisms. Detailed sequence available upon request.
Formulation
Liquid in 50 mM HEPES pH 7.5, + 100 mM NaCl + 5 mM DTT + 15 mM reduced glutathione + 20% glycerol
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Concentration
0.273 mg/ml
Source
Sf9 insect cells
Molecular Weight
GST-CDK2: 60.2 kDa GST-CCNA2: 74.9 kDa
Specific Activity
67 pmol/ug x min Method for determination of Km value & Specific activity: Assay conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 uM Na-orthovanadate, 1.2 mM DTT, 50 ug / ml PEG20.000, ATP (variable), Substrate: Histone H1, 20 ug / ml, Recombinant CDK2/CCNA2: 1.0 ug / ml Filter binding assay: MSFC membrane (Millipore)
Menge: 50 ug
Lieferbar: Out of stock
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Arbeitsanleitung

CS-CRC029.pdf