Recombinant Human CDK3/CCNE1 complex GST-His Active

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
ArtNr CS-CRC031
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Coexpression of human CDK3, amino acids M1-H305 (as in GenBank entry NM_001258)*, N-terminally fused to GST-HIS6-Thrombin cleavage site and Human CCNE1, amino acids M1-A395 (as in GenBank entry M73812)*, N-terminally fused to GST-Thrombin cleavage site. *Sequence may contain documented polymorphisms. Detailed sequence available upon request.
Formulation
Liquid in 50 mM Tris-HCl, pH 8.0 + 100 mM NaCl + 5 mM DTT + 15 mM reduced glutathione + 20% glycerol
Purification
Affinity purified using GSH-agarose
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Source
Sf9 cells
Molecular Weight
GST-CDK3: 67.99 kDa GST-CCNE1: 72.0 kDa
Specific Activity
112 pmol/ug x min Method for determination of Km value & Specific activity: Assay conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate, 1.2 mM DTT, 2.5 ug / 50 ul PEG20.000, ATP (variable), Substrate: Rb-CTF, 5 ug / 50 ul, Recombinant CDK3/CCNE1: 10 ng / 50 ul Filter binding assay, MAFC membrane (Millipore)

Arbeitsanleitung

CS-CRC031.pdf

Menge: 50 ug
Lieferbar: Out of stock
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