Recombinant Human CDK4/CCND1 complex (CycD1 complex) GST-His Active

Hersteller Cell Sciences
Typ Proteins
Specific against Human
Menge 50 ug
ArtNr CS-CRC033
eClass 6.1 34160400
eClass 9.0 42020190
Coexpression of human CDK4, amino acids S4-E303 (as in GenBank entry NM_000075)* and human CCND1, amino acids Q4-I295 (as in GenBank entry NM_053056)*, both N-terminally fused to GST-Thrombin cleavage site. *Sequence may contain documented polymorphisms. Detailed sequence available upon request.
Liquid in 50 mM HEPES, pH 7.5 + 100 mM NaCl + 5 mM DTT + 4 mM reduced glutathione + 20% glycerol
Affinity purified using GSH-agarose
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Sf9 cells
Molecular Weight
GST-CDK4: 59.7 kDa GST-CCND1: 59.99 kDa
Specific Activity
154 pmol/ug x min Method for determination of Km value & Specific activity: Assay conditions: 60 mM HEPES-NaOH, pH 7.5 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate 1.2 mM DTT 2.5 ug / 50 ul PEG20.000 ATP (variable) Substrate:Rb-CTF, 5 ug / 50 ul CDK4/CCND1: 200 ng / 50 ul Filter binding assay MSFC membrane (Millipore)
Menge: 50 ug
Lieferbar: Out of stock
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