Recombinant Human CDK5/CDK5R1 (p25) complex GST-His Active

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
Host Baculovirus
ArtNr CS-CRC036
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Coexpression of human CDK5, amino acids M1-P292 (as in GenBank NM_004935)*, N-terminally fused to GST-HIS6-Thrombin cleavage site and human p25NCK, amino acids A99-R307 (as in GenBank NM_003885)*, N-terminally fused to HIS6-Thrombin cleavage site. CDK5/p25NCK, SP007, was confirmed as CDK5/p25NCK by specific Western blot using anti CDK5 and p25NCK antibodies.
Formulation
Liquid in 50 mM Tris-HCl, pH 8.0 + 100 mM NaCl + 5 mM DTT + 4 mM reduced glutathione + 20% glycerol
Storage and Stability
Store at -80C. Avoid repeated freeze-thaw cycles.
Concentration
0.102 mg/ml (Bradford method using BSA as standard protein)
GeneID
1020 (CDK5), 8851 (p25NCK)
Source
Baculovirus infected Sf9 cells
Molecular Weight
62.841 kDa (GST-CDK5), 28.134 kDa (p25NCK)
Purity
One-step affinity purification using GSH-agarose
Specific Activity
16 pmol/ugxmin determined by assay: 60 mM HEPES-NaOH, pH 7.5 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate 1.2 mM DTT 2.5 ug / 50 ul PEG20, 000 ATP (variable) Substrate: Rb-CTF, 10 ug / 50 ul Recombinant CDK5/p25NCK: 200 ng / 50 ul Filter binding assay MSFC membrane
Protein Accession No
NP_004926 (CDK5), NP_003876 (p25NCK)
Menge: 50 ug
Lieferbar: Out of stock
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Arbeitsanleitung

CS-CRC036.pdf