Recombinant Human CDK6/CCND1 complex GST-His Active

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
Host Baculovirus
ArtNr CS-CRC038
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Co-expression of human CDK6, amino acids M1-A326 (as in GenBank entry NM_001259)* and human CycD1, amino acids Q4-I295 (as in GenBank entry NM_001758)*, both N-terminally fused to GST-Thrombin cleavage site. *Sequence may contain documented polymorphisms. Detailed sequence on request.
Formulation
50 mM Tris-HCl + pH 8.0 + 100 mM NaCl + 5 mM DTT + 15 mM reduced glutathione, 20% glycerol
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Concentration
0.725 ug/ul
GeneID
1021
Source
Baculovirus infected Sf9 cells
Molecular Weight
Theoretical MWGST-CDK6: 63, 279 Da Theoretical MWGST-CycD1: 59, 995 Da
Specific Activity
14 pmol/ugxmin Method for determination of Km value and specific activity: Assay conditions: 60 mM HEPES-NaOH, pH 7.5 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate 1.2 mM DTT 2.5 ug / 50 ul PEG20.000 ATP (variable) Substrate: Rb-CTF, 5 ug / 50 ul Recombinant CDK6/CyclinD1: 200 ng / 50 ul, Filter binding assay MAFC membrane (Millipore)
Protein Accession No
NM_001259
Menge: 50 ug
Lieferbar: Out of stock
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