Recombinant Human CDK9/CCNT1 complex (CycT complex) GST-His Active

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
Host Baculovirus
ArtNr CS-CRC041
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Coexpression of human CDK9, amino acids M1-F372 (as in GenBank entry NM_001261.2)*, N-terminally fused to GST-HIS6-Thrombin cleavage site and human CycT, amino acids M1-K726 (as in GenBank entry NM_001240.2)*, N-terminally fused to HIS6-Thrombin cleavage site *Sequence may contain documented polymorphisms, Detailed sequence on request
Formulation
50 mM Tris-HCl + pH 8.0 + 100 mM NaCl + 5 mM DTT + 4 mM reduced glutathione, 20% glycerol
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Concentration
0.168 ug/ul
GeneID
904
Source
Baculovirus infected Sf9 cells
Molecular Weight
Theoretical MWGST-CDK9: 72, 173 Da Theoretical MWCycT: 85, 441 Da
Specific Activity
26 pmol/ugxmin Method for determination of Km value and specific activity: Assay conditions: 660 mM HEPES-NaOH, pH 7.5 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate 1.2 mM DTT 2.5 ug / 50 ul PEG20.000 ATP (variable) Substrate: Rb-CTF, 10 ug / 50 ul Recombinant CDK9/CycT: 200 ng / 50 ul, Filter binding assay MSFC membrane (Millipore)
Protein Accession No
NM_001261.2
Menge: 50 ug
Lieferbar: Out of stock
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