Recombinant Human CSNK1A1/CK1 alpha 1 GST-His Active

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
Host Baculovirus
ArtNr CS-CRC042
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Human CK1-alpha1 Amino acids M1-F365 (as in GenBank entry DQ082865)*, N-terminally fused to GST-HIS6-Thrombin cleavage site. *Sequence may contain documented polymorphisms. Detailed sequence on request.
Formulation
50 mM Tris-HCl + pH 8.0 + 100 mM NaCl + 5 mM DTT + 4 mM reduced glutathione, 20% glycerol
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Concentration
0.058 ug/ul
GeneID
1452
Source
Baculovirus infected Sf9 cells
Molecular Weight
Theoretical MWFusion Protein: 71, 434 Da
Specific Activity
3 pmol/ugxmin Method for determination of Km value and specific activity: Assay conditions: 60 mM HEPES-NaOH, pH 7.5 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate 1.2 mM DTT 50 ug / ml PEG20.000 ATP (variable) Substrate: Casein, 50 ug / ml Recombinant CK1-alpha1: 400 ng / ml, Filter binding assay MSFC membrane (Millipore
Protein Accession No
DQ082865
Menge: 50 ug
Lieferbar: Out of stock
nicht lieferbar

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