Recombinant Human CK2 alpha 1 polypeptide Active *di

Hersteller Cell Sciences
Typ Proteins
Specific against Human
Menge 10 ug
ArtNr CS-CRC043
eClass 6.1 34160400
eClass 9.0 42020190
Casein kinase 2 (CK2) is a ubiquitous pleiotropic proliferation-associated serine/threonine protein kinase. The enzyme is probably present in all eukaryotic cells, implying that it has fundamental cellular functions. Similar to protein kinase A, CK2 is a tetramer containing 2 alpha- or alpha-prime subunits (or one of each) and 2 beta-subunits. The beta-subunit fills a regulatory role in the holoenzyme. The catalytic alpha-subunit corresponds to the C-subunit of PKA, the non-catalytic beta-subunit is unique and differs from the R-subunit of PKA in all known features. The alpha-/alpha-prime subunits are the catalytic subunits with distinct sequences and are encoded by different genes (Boldyreff et al. 1992, Wirkner et al. 1992, Yang-Feng et al. 1991). Whereas the CK2 alpha-subunit is found ubiquitously in all cells and organs the CK2 alpha-prime subunit is preferentially found in brain and testis (Guerra et al. 1999). Recently it was shown that the beta-subunit is trifunctional: (i) it confers stability to the holoenzyme, (ii) it increases enzyme activity and (iii) it determines substrate specificity. The human recombinant protein kinase CK2alpha1 was expressed in, E. coli, as a non fusion protein and purified by several chromatography steps. The alpha-subunit is constitutively active and suitable for labeling casein kinase 2 alpha substrates. It is shown to be extremely salt sensitive, the highest activity is shown without salt.
Supplied in 25 mM Tris-HCl, pH 8.5 + 500 mM NaCl + 1 mM DTT + 500 uM PMSF and 50% glycerol.
Storage and Stability
Quality guaranteed for 12 months. Store at -80C. Avoid freeze / thaw cycles
Molecular Weight
45.1 kDa
>95% by SDS-PAGE
Menge: 10 ug
Lieferbar: In stock
Listenpreis: 689,52 €
Preis: 689,52 €


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