Recombinant Human Mitogen-activated Protein kinase kinase kinase 8 His

Hersteller Cell Sciences
Kategorie Produkte / Proteins
Typ Proteins
Specific against Human
Menge 50 ug
Host Baculovirus
ArtNr CS-CRC048
Eclass 6.1 34160400
Eclass 9.0 42020190
Human COT, internal fragment, amino acids M30-R397 (as in GenBank entry NM_005204)*, N-terminal GST-HIS6 fusion protein (His tag) with a Thrombin cleavage site, expressed in Sf9 insect cells. *Sequence may contain documented polymorphisms. Detailed aa-sequence on request.
50 mM HEPES pH 7.5 + 100 mM NaCl + 5 mM DTT + 4 mM reduced glutathione, 20% glycerol
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
0.088 ug/ul
Baculovirus infected Sf9 cells
Molecular Weight
Theoretical MWFusion Protein: 71, 561 Da
Specific Activity
38 pmol/ugxmin Method for determination of Km value and specific activity: • Assay conditions: 60 mM HEPES-NaOH, pH 7.5 3 mM MgCl2 3 mM MnCl2 3 uM Na-orthovanadate 1.2 mM DTT 2.5 ug / 50 ul PEG20.000 ATP (variable) Substrate: Myelin Basic Protein, 5 ug / 50 ul COT: 200 ng / 50 ul • Filter binding assay MSFC membrane (Millipore)
Protein Accession No
Menge: 50 ug
Lieferbar: Out of stock
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