Native Human CAPN1

Hersteller Cell Sciences
Kategorie Produkte / Proteins
Typ Proteins
Specific against Human
Menge 5 ug
Host Human
ArtNr CS-CRC118B
Eclass 6.1 34160400
Eclass 9.0 42020190
CAPN1 consists of an 80 kDa large subunit and a 30 kDa small subunit. CAPN1 was purified by sequential chromatography through DEAE-Sepharose, A1.5 m Bio-Gel, and Phenyl-Sepharose CL-4B columns. Calpain’s activity is attributed to two main isoforms: u-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (u-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.
50 mM imidazole-HCl + 100mM NaCl + 5mM EGTA + 1mM DTT and 10% sucrose.
Storage and Stability
CAPN1 although stable at 10C for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Human Erythrocytes.
Greater than 90% as determined by SDS-PAGE.
Menge: 5 ug
Lieferbar: Out of stock
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