Recombinant Human Cytokeratin 14

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 1.0 mg
Host E.coli
ArtNr CS-CRC175C
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Cytokeratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. Cytokeratin 14 is a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
Formulation
Lyophilized from a sterile filtered solution containing 30 mM Tris-HCl, pH 8.0 + 9.5 M urea + 2 mM DTT + 2 mM EDTA + 10 mM methylammonium chloride
Reconstitution
Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.
Storage and Stability
Store lyophilized protein at -20C to -80C. Reconstituted protein is stable for 1 week at 2-4C. For long term storage, aliquot and store at -20C to -80C with a carrier protein (0.1% HSA or BSA) as a stabilizer. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
GeneID
3861
Source
E. coli
Molecular Weight
51.53 kDa
Purity
> 95% as determined by SDS-PAGE and RP-HPLC analyses
Protein Accession No
NP_001545
Endotoxin Level
< 0.1 ng/ug of KRT14
Menge: 1.0 mg
Lieferbar: In stock
Listenpreis: 6.880,51 €
Rabatt: -10,0%
Preis: 6.192,46 €
Sie sparen: 688,05 €
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Arbeitsanleitung

CS-CRC175C.pdf