Recombinant Human Collagen Type 1

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 10 mg
Host E.coli
ArtNr CS-CRC194
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Recombinant human collagen produced from, E. coli, is a non-glycosylated 96 kDa polypeptide chain. It is expressed with human collagen cDNA transcribed reversely from mRNA and purified by proprietary chromatographic techniques. Recombinant human Collage retains the function of collagen and unlike collagen extracted from animal tissues, rhCollagen is free from virus, water soluble, and with less immunogenecity for, in vivo, studies.
Formulation
Sterile filtered and lyophilized with no additives
Reconstitution
Centrifuge the vial prior to opening. Reconstitute the lyophilized rhCollagen in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted to other aqueous solutions and stored aliquoted at -20C for future use.
Storage and Stability
The lyophilized product is best stored at -20C. Reconstituted solution can be stored at 2-4C for 2-5 days. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA) and keep desiccated below -20C. Avoid freeze-thaw cycles.
Source
E. coli
Molecular Weight
96.0 kDa
Purity
> 95% as determined by SDS-PAGE and HPLC.
Endotoxin Level
Endotoxin level is less than 0.1 ng/mg of collagen
Biological Activity
When adding rhCollagen (>0.03% concentration) into culture plate, rhCollagen has remarkable effect on cell proliferation, improving 40-60% in comparison to control culture.

Arbeitsanleitung

CS-CRC194.pdf

Menge: 10 mg
Lieferbar: Out of stock
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