Recombinant Human CNTF

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 5 ug
Host E.coli
ArtNr CS-CRC400A
Eclass 6.1 34160400
Eclass 9.0 42020190
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Description
Ciliary Neurotrophic Factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. Recombinant Human CNTF is a single non-glycosylated polypeptide chain containing 200 amino acids.
Formulation
Lyophilized from a sterile filtered solution in PBS, pH 7.4
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
GeneID
1270
Source
E. coli
Molecular Weight
22.8 kDa
Purity
>97.0% by HPLC and SDS-PAGE
Specific Activity
>5 x 105 units/mg
Protein Accession No
NP_000605
Endotoxin Level
<0.1 ng/ug of CNTF
Biological Activity
Determined by the dose-dependant stimulation of TF-1 cells. The ED50 is < 2 ng/mL.
AA Sequence
MAFTEHSPLT PHRRDLCSRS IWLARKLRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
Menge: 5 ug
Lieferbar: In stock
Listenpreis: 206,00 €
Rabatt: -10,0%
Preis: 185,40 €
Sie sparen: 20,60 €
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Arbeitsanleitung

CS-CRC400A.pdf