Recombinant Human EGF

Hersteller Cell Sciences
Kategorie Produkte / Proteins
Typ Proteins
Specific against Human
Menge 1.0 mg
Host E.coli
ArtNr CS-CRE100C
Eclass 6.1 34160400
Eclass 9.0 42020190
Recombinant Human Epidermal Growth Factor (EGF) is a single non-glycosylated polypeptide chain containing 53 amino acids. Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the trans-membrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active.
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
E. coli
Molecular Weight
ca. 6.2 kDa
>98% by HPLC and SDS-PAGE
Specific Activity
> 1.0 x 10E7 IU/mg
Endotoxin Level
Less than 1EU/ug of rHuEGF as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using mouse Balb/c 3T3 cells is less than 0.1 ng/ml.
AA Sequence
Menge: 1.0 mg
Lieferbar: In stock
Listenpreis: 329,04 €
Rabatt: -10,0%
Preis: 296,14 €
Sie sparen: 32,90 €

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