Recombinant Human Erythropoietin alpha, Liquid

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 500 IU
Host CHO cells
ArtNr CS-CRE135A
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
This product is a liquid formulation of Recombinant Human Erythropoeitin alpha (EPO). EPO is a glycoprotein produced primarily by the kidney and is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The production of EPO by kidney cells is increased in response to hypoxia or anemia. Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure as well as for anemia of AZT treated AIDS patients. The cDNAs for EPO have been cloned from human, mouse, dog, etc. The mature proteins from the various species are highly conserved, exhibiting greater than 80% aa sequence identity. Human EPO cDNA encodes a 193 aa residue precursor protein that is processed to yield a 165 aa residue mature protein. EPO contains one O-linked and three N-linked glycosylation sites. Glycosylation of EPO is required for EPO biological activities, in vivo . EPO exhibits structural as well as aa sequence identity to the amino terminal 153 aa region of thrombopoietin.
Formulation
Sterile filtered clear colorless liquid in sodium citrate buffer + NaCl
Storage and Stability
Store at 2-4C under sterile conditions after opening. Stable for 2 years. Do not freeze.
Concentration
0.608 mg/ml
GeneID
2056
Source
CHO cells
Molecular Weight
Mature human EPO, containing 165 amino acid residues, has a predicted MW = approximately 21 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent MW = 36-40 kDa in SDS-PAGE.
Purity
>98% by SDS-PAGE and HPLC analyses
Specific Activity
Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocythemic mice and was found to be no less than 1.5 x105 IU/mg.
Endotoxin Level
< 0.1 ng/ug of protein.
AA Sequence
APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

Arbeitsanleitung

CS-CRE135A.pdf

Menge: 500 IU
Lieferbar: In stock
Listenpreis: 226,60 €
Preis: 226,60 €
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Arne Pelz

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