Recombinant Human FGF ac*dic

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 1.0 mg
Host E.coli
ArtNr CS-CRF000C
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Fibroblast Growth Factor acidic (FGF acidic), also known as FGF-1 and endothelial cell growth factor, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named “acidic fibroblast growth factor: (FGF1) and “basic fibroblast growth factor” (FGF2). Human FGF-acidic shares 54% amino acid sequence identity with FGF-basic. In mammalian FGF receptor family has 4 members, FGFR1, FGFR1, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types “b”, “c”. aFGF can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparin sulfate proteoglycan. FGF-acidic plays an important role in the regulation of cell survival, cell division, antiogenesis, cell differentiation and cell migration. FGF-acidic is also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion. Recombinant Human FGF acidic is a single, non-glycosylated polypeptide chain containing 141 amino acids.
Formulation
Lyophilized from a sterile filtered solution in PBS, pH 7.4
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. This depends upon the particular application employed. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
The lyophilized protein is stable at 2-8C, but should be kept desiccated at -20C for long term storage. Reconstituted protein is stable for 1 week at 2-8C. For maximal stability, divide the reconstituted protein into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
GeneID
2246
Source
E. coli
Molecular Weight
ca.15.8 kDa
Purity
>95% by SDS-PAGE and HPLC analyses.
Specific Activity
>3.3 x 10E6 IU/mg in the presence of 10 ug /ml heparin.
Endotoxin Level
<1 EU/ug as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50, determined by a cell proliferation assay using mouse BALB/c 3T3 cells is less than 0.3 ng/ml.
AA Sequence
MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Menge: 1.0 mg
Lieferbar: In stock
Listenpreis: 1.702,57 €
Preis: 1.702,57 €
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