Recombinant Human GCSF

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 10 ug
Host E.coli
ArtNr CS-CRG300B
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-a and IFN-g. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-a activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to mouse G-CSF and the two proteins show species cross-reactivity. Recombinant Human Granulocyte-Colony Stimulating Factor is a single non-glycosylated polypeptide chain containing 174 amino acids.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 10 mM sodium acetate buffer + 5% trehalose, pH 4.0.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
Stable at 2-8C, but best kept desiccated -20C. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
GeneID
1440
Source
E. coli
Molecular Weight
18.7 kDa
Purity
>98% by SDS-PAGE and HPLC
Specific Activity
>1 x 107 IU/mg
Endotoxin Level
Less than 1EU/ug as determined by LAL method.
Biological Activity
Recombinant Human G-CSF is fully biologically active when compared to standard. The ED50, determined by a cell proliferation assay using m, pise NFS-60 cells is less than 0.1 ng/ml.
AA Sequence
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
Menge: 10 ug
Lieferbar: In stock
Listenpreis: 349,81 €
Preis: 349,81 €
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