Recombinant Enhanced Green Fluorescent Protein His

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against other
Menge 100 ug
Host E.coli
ArtNr CS-CRE131B
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
The recombinant EGFP is expressed and purified from transformed, E. coli, using a method that ensures high purity and maximal GFP fluorescence. The recombinant EGFP is expressed and purified from transformed, E. coli, using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for, in vivo, injection or cell culture applications. The recombinant EGFP is a monomer with 265 amino acids tagged with 6x His on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody and GFP antibody on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea victoria, with Ex/Em = 488/507 nm (epsilon489 nm = 55, 000 M-1 Cm-1, epsilon280 nm = 21, 980 M-1 Cm-1).
Formulation
Lyophilized with no additives
Reconstitution
Centrifuge vial prior to opening. Reconstitute with PBS or appropriate buffer to a concentration of 1 mg/ml.
Storage and Stability
Store in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Concentration
1 mg/ml
Source
E. coli
Molecular Weight
32.7 kDa
Purity
97% by SDS PAGE and HPLC
Endotoxin Level
<=0.1 ng/ug of EGFP
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 804,78 €
Preis: 804,78 €
lieferbar

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