Recombinant E. coli GroEL/HSPD1

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against other
Menge 1.0 mg
Host E.coli
ArtNr CS-CRH109C
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
GroEL protein is the major heat shock protein of, E. coli, and belongs to the chaperonin(HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from, E. coli, and cloned into an expression vector. This protein was overexpressed in, E. coli, and was purified by using conventional chromatography techniques. Recombinant GroEL produced in, E. coli, is a single, non-glycosylated polypeptide chain conjtaining 548 amino acids and having a MW = 57.3 kDa.
Formulation
The protein (1 mg/ml) contains 25 mM Tris-HCl buffer pH 7.5 + 100 mM NaCl + 1 mM DTT + 10% Glycerol.
Storage and Stability
Store at 2-4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source
E. coli
Purity
Greater than 95.0% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE Coomassie.
Menge: 1.0 mg
Lieferbar: In stock
Listenpreis: 3.122,06 €
Preis: 3.122,06 €
lieferbar

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