Recombinant Carp Growth Hormone

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against other
Menge 10 ug
Host E.coli
ArtNr CS-CRH207A
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Growth-Hormone is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation, an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. Recombinant Carp Growth Hormone produced in, E. coli, is a single, non-glycosylated, polypeptide chain containing 188 amino acids & having a MW = 21, 408 Dalton. Carp Growth Hormone is purified by proprietary chromatographic techniques.
Formulation
The Carp GH was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8.
Storage and Stability
Lyophilized Carp Growth Hormone recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Carp Growth Hormone should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Source
E. coli
Molecular Weight
21, 408 Dalton
Purity
Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity
Carp GH is biologically active in rat 3T3 F442A preadipocytes, though its activity is 15-fold lower compared to bovine GH, but it is equally potent, in vivo, in promoting carp growth (Fine et al.1993). Furthermore, carp GH forms 1:2 complex with the extra cellular domain of ovine growth hormone receptor.
AA Sequence
The sequence of the first five N-terminal amino acids was determined and found to be Ser-Asp-Asn-Gln-Arg.
Menge: 10 ug
Lieferbar: In stock
Listenpreis: 226,60 €
Preis: 226,60 €
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