Recombinant Rat Stem Cell Factor

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Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Rat
Menge 2 ug
Host E.coli
ArtNr CS-CRS202A
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. In addition, it also promotes mast cell adhesion, migration, proliferation, and survival 3. Rat SCF shares 75%-90% a.a. sequence identity with canine, feline, mouse, and human SCF. Furthermore, rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage and Stability
This lyophilized preparation is stable at 2-4C, but should be kept desiccated at -20C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -80C. Avoid repeated freeze/thaw cycles.
GeneID
60427
Source
E. coli
Molecular Weight
Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.
Purity
>95% by SDS-PAGE and HPLC analyses.
Specific Activity
> 5 x 105 IU/mg
Protein Accession No
P21581
Endotoxin Level
Less than 1EU/ug as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using human TF-1 cells is less than 20 ng/ml.
AA Sequence
MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

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Menge: 2 ug
Lieferbar: In stock
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