ELISA Kit for Interleukin 6 (IL6)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Bovine
Applikationen ELISA
Menge 48T
ArtNr SEA079Bo-48
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Interleukin 6
Alternative Names
MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor
Uniprot
P26892
Detection range
7.8-500pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<3.0pg/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).
No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Cytokine, Tumor immunity, Infection immunity, Cardiovascular biology,
References
The Concentrations of Inflammatory Cytokines and Acute?Phase Proteins in the Peripheral Blood and Uterine Washings in Cows with Pyometra
Inflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum.
Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells
Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes
Inflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum
Inflammatory cytokine and acute phase protein concentrations in the peripheral blood and uterine washings of cows with subclinical endometritis in the late postpartum period
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Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells
Acute phase response in the primiparous dairy cows after repeated percutaneous liver biopsyduring the transition period.
Milk and serum IL-4, IL-6, IL-10, and amyloid A concentrations in cows with subclinical mastitis caused by coagulase-negative staphylococci
Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows
A study of altered cytokine rhythms associated with successful implantation in cows
Relationship between body condition score and plasma adipokines in early-lactating Holstein dairy cows
Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos …
MAVS induces a host cell defense to inhibit CSFV infection
Milk fever in dairy cows is preceded by activation of innate immunity and alterations in carbohydrate metabolism prior to disease occurrence
Effect of reproductive disorders on productivity and reproductive efficiency of dromedary she-camels in relation to cytokine concentration
Rifaximin anti?inflammatory activity on bovine endometrium primary cell cultures: a preliminary study

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Menge: 48T
Lieferbar: In stock
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