ELISA Kit for Interleukin 6 (IL6)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Gallus
Applikationen ELISA
Menge 48T
ArtNr SEA079Ga-48
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Interleukin 6
Alternative Names
MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor
Uniprot
Q90YI0
Detection range
15.6-1, 000pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<5.5pg/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).
No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area
Cytokine, Tumor immunity, Infection immunity, Cardiovascular biology,
References
The effect of different dietary levels of vitamin E and selenium on antioxidant status and immunological markers in serum of laying hens
Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo
Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expression
The effect of dietary methionine levels on fattening performance and selected blood and tissue parameters of turkeys
ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages
Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.
The effect of DL-, L-isomers and DL-hydroxy analog administered at 2 levels as dietary sources of methionine on the metabolic and antioxidant parameters and growth performance of turkeys
Redox and immunological status of turkeys fed diets with different levels and sources of copper

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Menge: 48T
Lieferbar: In stock
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