ELISA Kit for Folic Acid (FA)

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Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against General
Applikationen ELISA
Menge 5x96T
ArtNr CEA610Ge-5
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Folic Acid
Alternative Names
VB9, Vitamin B9, Folacin, Folate, V, Itamin M, Vitamin M, Vitamin Bc, Pteroyl-L-Glutamic Acid, Pteroyl-L-Glutamate
Detection range
39.1-10, 000pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<15.1pg/mL
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity
This assay has high sensitivity and excellent specificity for detection of Folic Acid (FA).
No significant cross-reactivity or interference between Folic Acid (FA) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folic Acid (FA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folic Acid (FA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add50µ Lstandardorsampletoeachwell.
        Andthenadd50µ LpreparedDetectionReagentAimmediately.
        Shakeandmix.Incubate1hourat37° C;
3.Aspirateandwash3times;
4.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
5.Aspirateandwash5times;
6.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
7.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Folic Acid (FA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Folic Acid (FA) and unlabeled Folic Acid (FA) (Standards or samples) with the pre-coated antibody specific to Folic Acid (FA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Folic Acid (FA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Folic Acid (FA) in the sample.
Research Area
Hematology, Reproductive science, Nutrition metabolism,
References
Vitamin Status and the Development of Postoperative Cognitive Decline in Elderly Surgical Oncologic Patients.
Development of Orally Applicable, Combinatorial Drug–Loaded Nanoparticles for the Treatment of Fibrosarcoma
Role of folic acid deficiency as a possible risk factor for erectile dysfunction
Low serum folic acid can be a potential independent risk factor for erectile dysfunction: a prospective case–control study

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Menge: 5x96T
Lieferbar: In stock
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