ELISA Kit for Melatonin (MT)

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Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against General
Applikationen ELISA
Menge 5x96T
ArtNr CEA908Ge-5
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Melatonin
Alternative Names
N-Acetyl-5-Methoxytryptamine
Detection range
12.35-1, 000pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<4.63pg/mL
Sample type
Serum, plasma and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity
This assay has high sensitivity and excellent specificity for detection of Melatonin (MT).
No significant cross-reactivity or interference between Melatonin (MT) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melatonin (MT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melatonin (MT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add50µ Lstandardorsampletoeachwell.
        Andthenadd50µ LpreparedDetectionReagentAimmediately.
        Shakeandmix.Incubate1hourat37° C;
3.Aspirateandwash3times;
4.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
5.Aspirateandwash5times;
6.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
7.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Melatonin (MT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Melatonin (MT) and unlabeled Melatonin (MT) (Standards or samples) with the pre-coated antibody specific to Melatonin (MT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Melatonin (MT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Melatonin (MT) in the sample.
Research Area
Metabolic pathway, Tumor immunity, Neuro science, Hormone metabolism,
References
Effects of melatonin on islet neogenesis and beta cell apoptosis in streptozotocin-induced diabetic rats: an immunohistochemical study
Biomarkers inducing changes due to microwave exposure effect on rat brain
Comparison of the flow characteristics for the honeycomb and labyrinth seal using numerical simulation
The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field
Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in rats
Improvement of oxidative stress and immunity by melatonin: An age dependent study in golden hamster
Melatonin and its correlation with testosterone in polycystic ovarian syndrome
Daily variation in melatonin level, antioxidant activity and general immune response of peripheral blood mononuclear cells and lymphoid tissues of Indian goat Capra …
Serum Melatonin Level Disturbance is Related to Metabolic Syndrome and Subclinical Arterial Dysfunction in Shift Working Healthy Men
Melatonin Administration Aids Bone Healing in Diabetic Rats
Photoperiodic induced melatonin regulates immunity and expression pattern of melatonin receptor MT1 in spleen and bone marrow mononuclear cells of male golden hamster.
Influence of?circadian?time?and lighting conditions on expression of melatonin?receptors?1?and?2?in?murinelymphocytes.
Molecularly imprinted electrochemical sensing of urinary melatonin in a microfluidic system
Localization and expression of androgen receptor (AR) and melatonin membrane receptor (MT1R) in accessory sex organs of adult male golden hamster, Mesocricetus auratus: Modulation by photoperiod.
Titanium oxide (TiO2) nanoparticles in induction of apoptosis and inflammatory response in brain
MT1 receptor expression and AA-NAT activity in lymphatic tissue following melatonin administration in male golden hamster
Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus
Melatonin prevents dexamethasone-induced testicular oxidative stress and germ cell apoptosis in golden hamster, Mesocricetus auratus

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Menge: 5x96T
Lieferbar: In stock
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