ELISA Kit for Laminin (LN)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 10x96T
ArtNr SEA082Hu-10
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Laminin
Alternative Names
LAM
Detection range
7.8-500ng/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<2.8ng/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Laminin (LN).
No significant cross-reactivity or interference between Laminin (LN) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin (LN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin (LN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin (LN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin (LN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin (LN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin (LN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
References
Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton' s jelly
Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media
Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progression
Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis.
Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas
Clinical significance of serum laminin and typea€‘IV collagen levels in cutaneous melanoma patients
Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.
Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Response
High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects

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Menge: 10x96T
Lieferbar: In stock
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