ELISA Kit for Interleukin 12 (IL12)

Hersteller Cloud-Clone
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 48T
ArtNr SEA111Hu-48
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Target Name
Interleukin 12
Detection range
3.12-200pg/mL
Application info
Enzyme-linked immunosorbent assay for Antigen Detection.
Sensitivity
<1.31pg/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
3h
Method
Double-antibody Sandwich
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 12 (IL12).
No significant cross-reactivity or interference between Interleukin 12 (IL12) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1.Prepareallreagents, samplesandstandards;
2.Add100µ Lstandardorsampletoeachwell.Incubate2hoursat37° C;
3.Aspirateandadd100µ LpreparedDetectionReagentA.Incubate1hourat37° C;
4.Aspirateandwash3times;
5.Add100µ LpreparedDetectionReagentB.Incubate30minutesat37° C;
6.Aspirateandwash5times;
7.Add90µ LSubstrateSolution.Incubate10-20minutesat37° C;
8.Add50µ LStopSolution.Readat450nmimmediately.
Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to IL12A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL12B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
References
A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme …
Antitumor efficacy of the Runx2?dendritic cell vaccine in triple?negative breast cancer in vitro
Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-gamma in …
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RBP and Alb in neonatal hydronephrosis and its association with IL-12 levels in pregnant women before delivery

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Menge: 48T
Lieferbar: In stock
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